Research topics & objectives
The laboratory aims to establish correlations between molecular data and the phenotype / clinical action of lymphomas: prognostic or theranostic value; implementation of diagnostic tests; identification of the biological value of the variants identified in the tumour and plasma.Research topics
Lymphoma genomics and transcriptomics
Lymphoma sub-types
- B-cell lymphomas
- Diffuse large B-cell lymphomas
- Hodgkin lymphomas
- Primary central nervous system lymphomas
- Mantle-cell lymphomas
Key words
Genome, Genotyping, Transcriptomics, Immunoglobulin genes, Transfer, Methylation

Fabrice
Jardin
U1245

expertise
BIOLOGICAL TARGETS AND IN VITRO/IN VIVO/EX VIVO MODELS
Targets
Molecular studies of the BCR component coding genes and VH genes in particular, driver genes (MYD88), cell cycle genes (CCND), XPO
Models:
- Xenografts on mice
- Mantle-cell lymphoma lineages, modified to express the GFP and/or luciferase (REC1, Granta-519, Z138 and JeKo1)
- JeKo1 et Z138 lineages resistant to bortezomib (JBR and ZBR)
BLOOD AND TISSUE BIOMARKERS
- Study of the recurring mutations and anomalies of the number of gene copies with a prognostic impact
- Targeted medium-throughput transcriptomic studies through RT-MLPA and transfer to immunohistochemistry
EARLY ACTIVITY PHARMACODYNAMIC SIGNALS
Correlation between transcriptomics/genomics and PETScan imaging
TOOLS, PROCESSES AND PLATFORMS LINKED TO THE CLINICAL RESEARCH
- Medium throughput genotyping platform, multiplex PCR analysis, study of the methylation of key genes through pyrosequencing
- Tumour bank and cold centre
- Conventional cytogenetics and FISH
- Clinical research unit with promotion centre and early stage trial platform
INNOVATING TECHNOLOGY
- RT-MLPA
- Cell-free DNA: characterisation of the tumour cell-free DNA circulating in the plasma
- Liquid biopsy
- Proximity ligation assay
- CRISPR-barcoding, CRISPR/Cas9
Platforms & technical resources
- Sequencers (PGM), quantitative PCR (TaqMan), pyrosequencer
- Medium throughput genotyping and transcriptomic analysis platform (Illumina, BeadExpress)
- Flow cytometry
- Cell transfection (Amaxa), cloning, cell culture
- Tissue-micro-array
- Bioinformatics
- Nanostring (Ncounter)
- Digital PCR (Stilla)
R&D offer
The laboratory offers collaborative projects on:
- the quantification of recurring mutations in plasma DNA
- anatomical-clinical / molecular correlations and TEP scan imaging.
- bioinformatics tools for analysing variants and their integration in routine diagnostics
- the development of tools for the phenotyping of B and T-cell lymphomas.