Research objectives and themes
Understand how the tumor cells of certain B and T lymphomas acquire or divert various immune functions in interaction with their micro-environment.Research Themes
The team is studying the transcriptional heterogeneity of lymphoma cells and their immune microenvironment, using single-cell genomics approaches combined with large-scale bioinformatics analyzes.
Keywords
Germinal centers, follicular lymphoma (FL), diffuse large cell B lymphoma (DLBCL), angioimmunoblastic T lymphoma (AITL), single-cell RNA-seq, transcriptional heterogeneity, cell differentiation

Pierre
Milpied
Team Leader
Centre d’Immunologie de Marseille-Lumigny (CIML)
“Belonging to the CALYM consortium means benefiting from the scientific, clinical and translational expertise of partner teams and CALYM staff, and accessing the exceptional resources of the CeVi collection. Support and essential tools to collectively contribute to lymphoma research. ”

Expertise
- Mouse antigen-specific B immune response models
- Models of ex vivo activation of B lymphocytes and high-throughput transcriptional analysis (RNA-seq, scRNA-seq)
- Analysis / Sorting of sub-populations of primary tumor cells by flow cytometry
- Analysis / Sorting of the immune micro-environment by flow cytometry
- Integrative high-throughput analyzes on single cells (phenotype + transcriptome + BCR / TCR repertoire) by single-cell RNA-seq
- Bioinformatic analyzes of high-speed multi-modal data on single cells
Technical platforms and resources
- Genomics, Bioinformatics and Cytometry platforms of the Marseille-Luminy Center for Immunology (CIML)
TOP 5 of the latest scientific publications
Calym
FB5P-seq: FACS-based 5-prime end single-cell RNA-seq for integrative analysis of transcriptome and antigen receptor repertoire in B and T cells.
Front Immunol. 2020 Mar 3;11:216. doi: 10.3389/fimmu.2020.00216.
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Calym
Human germinal center transcriptional programs are de-synchronized in B cell lymphoma.
Nat Immunol. 2018 Sep;19(9):1013-1024. doi: 10.1038/s41590-018-0181-4.
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Calym
High-dimensional single-cell analysis identifies organ-specific signatures and conserved NK cell subsets in humans and mice.
. Immunity. 2018 Nov 20;49(5):971-986.e5. doi: 10.1016/j.immuni.2018.09.009.
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Calym
Premalignant cell dynamics in indolent B-cell malignancies
Curr Opin Hematol. 2015 Jul;22(4):388-96. doi: 10.1097/MOH.0000000000000159.
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Calym
Class-switched memory B cells remodel BCRs within secondary germinal centers.
Nat Immunol. 2015 Mar;16(3):296-305. doi: 10.1038/ni.3095.
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