Maintenance of genome integrity during DNA replication / Epigenetic changes and genetic instability in normal and tumor B cells.

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Research Objectives and Themes

Understanding the molecular mechanisms of cancer for better management of the disease

Objectives

Study tumorigenesis, understand the mechanisms of progression and drug resistance to develop new diagnostic and treatment strategies.

Keywords

Mechanisms of response to replicative stress and resistance to treatment, interference between replication and transcription, replicative stress and immune response, genetic instability, epigenetic regulation, multi-omics, biomarkers , precision medicine.

Jerome Moreaux Experts Recherche Lymphome
Jérôme Moreaux
Philippe Pasero Experts Recherche Lymphome
Philippe Pasero

” Being part of CALYM allows us to participate in a collaborative network of dynamic and complementary research teams and accelerate the development and enhancement / transfer of team innovations in the field of lymphoma. “

 Jérôme Moreaux
Expertise

Targets

 

  • Epigenetic regulation.
  • Response to DNA damage.
  • Inflammatory response induced by DNA damage

Models

  • In vitro: human lines of diffuse large B cell lymphoma, of mantle cell lymphoma including the characterization of the response to several treatments and the molecular characterization.
  • Ex vivo: Co-cultures of primary B lymphoma cells with cells from the microenvironment.
  • Molecular characterization (RNA-seq; Exome-seq) of patient cohorts including clinical and biological data.

Biomarkers

Prognostic and Theranostic biomarkers for treatments targeting the response to DNA damage, epigenetic changes and iron metabolism in B cell lymphomas.

Innovative technologies

  • Primary and normal B cell transduction tools.
  • Multi-omic analysis strategies for the development of prognostic / theranostic biomarkers.
  • Methods of analysis of replicative stress (analysis of isolated DNA fibers, mapping of DNA replication and chromosome breaks, replication-transcription conflicts, etc.)
  • Analysis of cytosolic DNA and circulating DNA generated by replicative stress

Technical platforms and resources

 

  • Cellular imaging platform (Institute of Human Genetics)
  • Genomics platform – Montpellier GenomiX
  • Bioinformatics and multi-omic analyzes (Institute of Human Genetics)
  • Flow cytometry (CHU Montpellier)

Top 5 des dernières publications scientifiques

Calym 2019_Overexpression of Claspin and Timeless protects cancer cells from replication stress in a checkpoint-independent manner.
Bianco JN, Bergoglio V, Lin YL, Pillaire MJ, Schmitz AL, Gilhodes J, Lusque A, Mazières J, Lacroix-Triki M, Roumeliotis TI, Choudhary J, Moreaux J, Hoffmann JS, Tourrière H* and Pasero P*
Nat Commun, 10, 910. Learn more
Calym 2019_EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation.
Herviou L, Jourdan M, Martinez AM, Cavalli G, Moreaux J.
Leukemia. 2019. Aug;33(8):2047-2060. Learn more
Calym 2018_SAMHD1 acts at stalled replication forks to prevent induction of interferon.
Coquel F#, Silva M-J#, Técher H#, Zadorozhny K, Sushma S, Nieminuszczy J, Mettling C, Dardillac E, Barthe A, Schmitz AL, Promonet A, Cribier A, Sarrazin A, Niedzwiedz W, Lopez B, Costanzo V, Krejci L, Chabes A, Benkirane M, Lin YL* and Pasero P*
Nature, 557, 57-61. Learn more
Calym 2017_Global miRNA expression analysis identifies novel key regulators of plasma cell differentiation and malignant plasma cell.
Kassambara A, Jourdan M, Bruyer A, Robert N, Pantesco V, Elemento O, Klein B, Moreaux J.
2017. Jun 2;45(10):5639-5652. Learn more
Calym 2017_RECQ1 helicase is involved in replication stress survival and drug resistance in multiple myeloma.
Viziteu E, Klein B, Basbous J, Lin YL, Hirtz C, Gourzones C, Tiers L, Bruyer A, Vincent L, Grandmougin C, Seckinger A, Goldschmidt H, Constantinou A, Pasero P, Hose D and Moreaux J
2017. Jun 2;45(10):5639-5652. Learn more